EFFECT OF SILIBININ ON THE SENSITIVITY OF ENTEROBACTER CLOACAE RESISTANT ISOLATE TO GENTAMICIN: IN VITRO STUDY

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Abstract – The present study was designed to evaluate the influence of silibinin on the gentamicin activity
against the resistant strain of Enterobacter cloacae. A gentamicin resistant isolate of Enterobacter cloaca was
obtained from Al-Sheikh Zayed hospital, Baghdad and cultivated in Mueller Hinton agar; the impact of
different concentrations of gentamicin, silibinin or their combination were determined using the
conventional drug sensitivity assay to determine the growth inhibitory parameters of each. The study
revealed strong resistance for the pathogen against gentamicin and very poor sensitivity for silibinin at the
highest concentration used. The isobologram technique was followed to determine the interaction of both
drugs against the pathogen. Silibinin antagonizes the gentamicin effect when combined at 7:3, 5:5 and 3:7
ratios. In conclusion, silibinin can augment resistance of Enterobacter cloacae to gentamicin when coadministered as a combination. Further studies are recommended to determine the precise mechanism
through which gentamicin resistance was conferred and the way through which silibinin antagonized
gentamicin.

Key words : Enterobacter cloacae, Silibinin, Gentamicin, Bacterial resistance

DOI link: EFFECT OF SILIBININ ON THE SENSITIVITY OF ENTEROBACTER CLOACAE RESISTANT ISOLATE TO GENTAMICIN: IN VITRO STUDYhttp://www.envirobiotechjournals.com/article_abstract.php?aid=10566&iid=304&jid=1